PLoS ONE (Jan 2011)

Mediator subunit 12 is required for neutrophil development in zebrafish.

  • Maria-Cristina Keightley,
  • Judith E Layton,
  • John W Hayman,
  • Joan K Heath,
  • Graham J Lieschke

DOI
https://doi.org/10.1371/journal.pone.0023845
Journal volume & issue
Vol. 6, no. 8
p. e23845

Abstract

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Hematopoiesis requires the spatiotemporal organization of regulatory factors to successfully orchestrate diverse lineage specificity from stem and progenitor cells. Med12 is a regulatory component of the large Mediator complex that enables contact between the general RNA polymerase II transcriptional machinery and enhancer bound regulatory factors. We have identified a new zebrafish med12 allele, syr, with a single missense mutation causing a valine to aspartic acid change at position 1046. Syr shows defects in hematopoiesis, which predominantly affect the myeloid lineage. Syr has identified a hematopoietic cell-specific requirement for Med12, suggesting a new role for this transcriptional regulator.