Drug Design, Development and Therapy (Jul 2023)
The First Discovery of a Microstructure in Black Plaster and Its Performance Characterization
Abstract
Yingpeng Li,1 Ting Zhu,2,3 Jingxian Liang,2 Yuanqun Wu,2 Xiantong Guan,2 Tingting Liu,2 Shaowa Lü,2 Yongji Li,2 Yanhong Wang,2 Yang Ping4 1College of Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, People’s Republic of China; 2Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese Medicine), Ministry of Education, Harbin, 150040, People’s Republic of China; 3Center of Pharmaceutical Engineering and Technology, College of Pharmacy, Harbin University of Commerce, Harbin, 150076, People’s Republic of China; 4College of Pharmacy, Jiamusi University, Jiamusi, Heilongjiang, 154007, People’s Republic of ChinaCorrespondence: Yanhong Wang, Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese Medicine), Ministry of Education, Harbin, 150040, People’s Republic of China, Tel +86 18845587616, Email [email protected] Yang Ping, College of Pharmacy, Jiamusi University, Jiamusi, Heilongjiang, 154007, People’s Republic of China, Tel +86 18245482328, Email [email protected]: Black plaster is one of the classic dosage forms of traditional Chinese medicine for external use and has been widely utilized since the Tang and Song Dynasties. In this paper, we take Goupi Gao as the research object and discuss the scientific characteristics of the black plaster dosage form. Goupi Gao ointment is a plaster for external use of traditional Chinese medicine.Methods: Methods for the morphological and quantitative characterization of black plaster’s microstructure, based on FESEM-IPP (Field Emission Scanning Electron Microscope IPP Image Processing) technology, were established. According to the actual operating temperature of Goupi Gao, three temperatures were selected: 28°C, 35°C, and 45°C. A UPLC analysis method was applied to the cinnamaldehyde and eugenol in Goupi Gao, and the release behavior of Goupi Gao from three samples at three temperatures was investigated using the paddle over disk method. Preparation of rabbit model of knee osteoarthritis of cold blood stasis type by cold stimulation combined with drug induction.Results: In terms of morphology, Goupi Gao and the blank black plaster matrix both formed a double continuous phase system with a thicker vegetable oil phase and crossed “branched” soap crystal fibers. Based on the IPP image quantification parameters, the pore area (A) was highly positively correlated with temperature. After the 28 °C treatment, A1 = (216.8± 59.5) μm2; after the 35 °C treatment, A2 = (259.7± 52.8) μm2; after the 45 °C treatment, A3 = (408.0± 57.7) μm2, and there were no significant differences in other pore parameters.Conclusion: The black plaster matrix’s unique structure makes it highly applicable in numerous medications; it exhibits slow-release and performs well in extreme temperatures, with good adhesion and peeling properties.Keywords: black plaster, Goupi Gao, microstructure, quantitative characterization, release behavior, pharmacodynamics
