The NAC transcription factor MdNAC29 negatively regulates drought tolerance in apple

Sen Li; Sen Li; Sen Li; Xiuli Jing; Xiuli Jing; Xiuli Jing; Qiuping Tan; Qiuping Tan; Qiuping Tan; Binbin Wen; Binbin Wen; Binbin Wen; Xiling Fu; Xiling Fu; Xiling Fu; Dongmei Li; Dongmei Li; Dongmei Li; Xiude Chen; Xiude Chen; Xiude Chen; Wei Xiao; Wei Xiao; Wei Xiao; Ling Li; Ling Li; Ling Li

doi:10.3389/fpls.2023.1173107

Frontiers in Plant Science (Jul 2023)

The NAC transcription factor MdNAC29 negatively regulates drought tolerance in apple

Sen Li,
Sen Li,
Sen Li,
Xiuli Jing,
Xiuli Jing,
Xiuli Jing,
Qiuping Tan,
Qiuping Tan,
Qiuping Tan,
Binbin Wen,
Binbin Wen,
Binbin Wen,
Xiling Fu,
Xiling Fu,
Xiling Fu,
Dongmei Li,
Dongmei Li,
Dongmei Li,
Xiude Chen,
Xiude Chen,
Xiude Chen,
Wei Xiao,
Wei Xiao,
Wei Xiao,
Ling Li,
Ling Li,
Ling Li

Affiliations

Sen Li: College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
Sen Li: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
Sen Li: Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, Shandong Agricultural University, Tai’an, China
Xiuli Jing: College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
Xiuli Jing: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
Xiuli Jing: Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, Shandong Agricultural University, Tai’an, China
Qiuping Tan: College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
Qiuping Tan: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
Qiuping Tan: Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, Shandong Agricultural University, Tai’an, China
Binbin Wen: College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
Binbin Wen: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
Binbin Wen: Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, Shandong Agricultural University, Tai’an, China
Xiling Fu: College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
Xiling Fu: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
Xiling Fu: Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, Shandong Agricultural University, Tai’an, China
Dongmei Li: College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
Dongmei Li: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
Dongmei Li: Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, Shandong Agricultural University, Tai’an, China
Xiude Chen: College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
Xiude Chen: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
Xiude Chen: Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, Shandong Agricultural University, Tai’an, China
Wei Xiao: College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
Wei Xiao: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
Wei Xiao: Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, Shandong Agricultural University, Tai’an, China
Ling Li: College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
Ling Li: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
Ling Li: Shandong Collaborative Innovation Center for Fruit and Vegetable Production with High Quality and Efficiency, Shandong Agricultural University, Tai’an, China

DOI: https://doi.org/10.3389/fpls.2023.1173107
Journal volume & issue: Vol. 14

Abstract

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Drought stress is an adverse stimulus that affects agricultural production worldwide. NAC transcription factors are involved in plant development and growth but also play different roles in the abiotic stress response. Here, we isolated the apple MdNAC29 gene and investigated its role in regulating drought tolerance. Subcellular localization experiments showed that MdNAC29 was localized to the nucleus and transcription was induced by the PEG treatment. Over-expression of MdNAC29 reduced drought tolerance in apple plants, calli, and tobacco, and exhibited higher relative conductivity, malondialdehyde (MDA) content, and lower chlorophyll content under drought stress. The transcriptomic analyses revealed that MdNAC29 reduced drought resistance by modulating the expression of photosynthesis and leaf senescence-related genes. The qRT-PCR results showed that overexpression of MdNAC29 repressed the expression of drought-resistance genes. Yeast one-hybrid and dual-luciferase assays demonstrated that MdNAC29 directly repressed MdDREB2A expression. Moreover, the yeast two-hybrid and bimolecular fluorescence complementation assays demonstrated that MdNAC29 interacted with the MdPP2-B10 (F-box protein), which responded to drought stress, and MdPP2-B10 enhanced the repressive effect of MdNAC29 on the transcriptional activity of the MdDREB2A. Taken together, our results indicate that MdNAC29 is a negative regulator of drought resistance, and provide a theoretical basis for further molecular mechanism research.

Published in Frontiers in Plant Science

ISSN: 1664-462X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: https://www.frontiersin.org/journals/plant-science

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