Isolation, genetic identification and degradation characteristics of COD-degrading bacterial strain in slaughter wastewater

Wen Li; Ming-Xi Jia; Jing Deng; Jian-Hui Wang; Qin-Lu Lin; Cun Liu; Sha-Sha Wang; Jian-Xin Tang; Xiao-Xi Zeng; Liang Ma; Wei Su; Xue-Ying Liu; Fang Cai; Li-Yi Zhou

Saudi Journal of Biological Sciences (Dec 2018)

Isolation, genetic identification and degradation characteristics of COD-degrading bacterial strain in slaughter wastewater

Wen Li,
Ming-Xi Jia,
Jing Deng,
Jian-Hui Wang,
Qin-Lu Lin,
Cun Liu,
Sha-Sha Wang,
Jian-Xin Tang,
Xiao-Xi Zeng,
Liang Ma,
Wei Su,
Xue-Ying Liu,
Fang Cai,
Li-Yi Zhou

Affiliations

Wen Li: Key Laboratory of Biological Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China; National Engineering Laboratory for Rice and Byproducts Deep Processing, College of Food, Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China
Ming-Xi Jia: Key Laboratory of Biological Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China
Jing Deng: Key Laboratory of Biological Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China; National Engineering Laboratory for Rice and Byproducts Deep Processing, College of Food, Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China; Key Laboratory of Advanced Packaging Materials and Technology, College of Packaging and Material Engineering, Hunan University of Technology, Zhuzhou 412007, China; Corresponding author at: Key Laboratory of Biological Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China.
Jian-Hui Wang: School of Chemistry and Bioengineering, Changsha University of Science and Technology, Changsha 410114, China
Qin-Lu Lin: National Engineering Laboratory for Rice and Byproducts Deep Processing, College of Food, Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China
Cun Liu: Key Laboratory of Biological Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China
Sha-Sha Wang: Key Laboratory of Biological Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China
Jian-Xin Tang: Key Laboratory of Biological Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China
Xiao-Xi Zeng: Key Laboratory of Biological Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China
Liang Ma: Key Laboratory of Biological Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China
Wei Su: Key Laboratory of Biological Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China
Xue-Ying Liu: Key Laboratory of Biological Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China
Fang Cai: School of Foreign Language, Hunan University of Technology, Zhuzhou 412007, China
Li-Yi Zhou: Key Laboratory of Biological Nanomaterials and Devices, College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, China; National Engineering Laboratory for Rice and Byproducts Deep Processing, College of Food, Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China

Journal volume & issue: Vol. 25, no. 8
pp. 1800 – 1805

Abstract

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Contamination of water by meat production is an important and extensive environmental problem and even threat to human health. Biodegradation is a major mechanism which removes the pollutants from the environment. Therefore, the present study aimed to isolate and characterize a COD degrading bacteria which can effectively degrade slaughter wastewater. Six COD degrading bacteria were isolated from slaughtering waste water and sludge in Hunan a meat product Co., Ltd. And the COD degradation rate of each strain was determined by potassium permanganate method. Through observing morphologically and analyzing sequence to 16S rDNA, the highest COD degradation strain was Bacillus velezensis by preliminarily identified and classified, reaching 11.80%. The suitable conditions of the growth of Bacillus velezensis strain were 37 °C, pH 7.0, the peptone concentration 1.5%, and the yeast extract concentration 0.8%. Keywords: Degrading bacteria, Bacillus velezensis, Identification, Characterization, Slaughter wastewater

Published in Saudi Journal of Biological Sciences

ISSN: 1319-562X (Print)
Publisher: Elsevier
Country of publisher: Saudi Arabia
LCC subjects: Science: Biology (General)
Website: http://www.journals.elsevier.com/saudi-journal-of-biological-sciences/

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