The non-catalytic role of DNA polymerase epsilon in replication initiation in human cells

Sameera Vipat; Dipika Gupta; Sagun Jonchhe; Hele Anderspuk; Eli Rothenberg; Tatiana N. Moiseeva

doi:10.1038/s41467-022-34911-4

Nature Communications (Nov 2022)

The non-catalytic role of DNA polymerase epsilon in replication initiation in human cells

Sameera Vipat,
Dipika Gupta,
Sagun Jonchhe,
Hele Anderspuk,
Eli Rothenberg,
Tatiana N. Moiseeva

Affiliations

Sameera Vipat: Department of Chemistry and Biotechnology, Tallinn University of Technology
Dipika Gupta: Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine
Sagun Jonchhe: Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine
Hele Anderspuk: Department of Chemistry and Biotechnology, Tallinn University of Technology
Eli Rothenberg: Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine
Tatiana N. Moiseeva: Department of Chemistry and Biotechnology, Tallinn University of Technology

DOI: https://doi.org/10.1038/s41467-022-34911-4
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 15

Abstract

Read online

DNA polymerase epsilon has a critical role in DNA replication initiation. Here, the authors show that in human cancer cells POLE is dispensable for the replicative helicase assembly but not for replication initiation, which requires the non-catalytic domain of POLE1.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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