Accurate immune repertoire sequencing reveals malaria infection driven antibody lineage diversification in young children

Ben S. Wendel; Chenfeng He; Mingjuan Qu; Di Wu; Stefany M. Hernandez; Ke-Yue Ma; Eugene W. Liu; Jun Xiao; Peter D. Crompton; Susan K. Pierce; Pengyu Ren; Keke Chen; Ning Jiang

doi:10.1038/s41467-017-00645-x

Nature Communications (Sep 2017)

Accurate immune repertoire sequencing reveals malaria infection driven antibody lineage diversification in young children

Ben S. Wendel,
Chenfeng He,
Mingjuan Qu,
Di Wu,
Stefany M. Hernandez,
Ke-Yue Ma,
Eugene W. Liu,
Jun Xiao,
Peter D. Crompton,
Susan K. Pierce,
Pengyu Ren,
Keke Chen,
Ning Jiang

Affiliations

Ben S. Wendel: McKetta Department of Chemical Engineering, Cockrell School of Engineering, University of Texas at Austin
Chenfeng He: Department of Biomedical engineering, Cockrell School of Engineering, University of Texas at Austin
Mingjuan Qu: Department of Biomedical engineering, Cockrell School of Engineering, University of Texas at Austin
Di Wu: Department of Biomedical engineering, Cockrell School of Engineering, University of Texas at Austin
Stefany M. Hernandez: McKetta Department of Chemical Engineering, Cockrell School of Engineering, University of Texas at Austin
Ke-Yue Ma: Institute for Cellular and Molecular Biology, College of Natural Sciences, University of Texas at Austin
Eugene W. Liu: Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Jun Xiao: ImmuDX, LLC
Peter D. Crompton: Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Susan K. Pierce: Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health
Pengyu Ren: Department of Biomedical engineering, Cockrell School of Engineering, University of Texas at Austin
Keke Chen: Department of Computer Science and Engineering, Wright State University
Ning Jiang: Department of Biomedical engineering, Cockrell School of Engineering, University of Texas at Austin

DOI: https://doi.org/10.1038/s41467-017-00645-x
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 14

Abstract

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Somatic hypermutation of antibodies can occur in infants but are difficult to track. Here the authors present a new method called MIDCIRS for deep quantitative repertoire sequencing with few cells, and show infants as young as 3 months can expand antibody lineage complexity in response to malaria infection.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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