Nature Communications (Nov 2019)
7-Deazaguanine modifications protect phage DNA from host restriction systems
- Geoffrey Hutinet,
- Witold Kot,
- Liang Cui,
- Roman Hillebrand,
- Seetharamsingh Balamkundu,
- Shanmugavel Gnanakalai,
- Ramesh Neelakandan,
- Alexander B. Carstens,
- Chuan Fa Lui,
- Denise Tremblay,
- Deborah Jacobs-Sera,
- Mandana Sassanfar,
- Yan-Jiun Lee,
- Peter Weigele,
- Sylvain Moineau,
- Graham F. Hatfull,
- Peter C. Dedon,
- Lars H. Hansen,
- Valérie de Crécy-Lagard
Affiliations
- Geoffrey Hutinet
- Department of Microbiology and Cell Science, University of Florida
- Witold Kot
- Department of Environmental Science, Aarhus University
- Liang Cui
- Singapore-MIT Alliance for Research and Technology, Antimicrobial Resistance Interdisciplinary Research Group, Campus for Research Excellence and Technological Enterprise
- Roman Hillebrand
- Department of Biological Engineering and Center for Environmental Health Sciences, Massachusetts Institute of Technology
- Seetharamsingh Balamkundu
- Singapore-MIT Alliance for Research and Technology, Antimicrobial Resistance Interdisciplinary Research Group, Campus for Research Excellence and Technological Enterprise
- Shanmugavel Gnanakalai
- Singapore-MIT Alliance for Research and Technology, Antimicrobial Resistance Interdisciplinary Research Group, Campus for Research Excellence and Technological Enterprise
- Ramesh Neelakandan
- Singapore-MIT Alliance for Research and Technology, Antimicrobial Resistance Interdisciplinary Research Group, Campus for Research Excellence and Technological Enterprise
- Alexander B. Carstens
- Department of Environmental Science, Aarhus University
- Chuan Fa Lui
- School of Biological Sciences, Nanyang Technological University
- Denise Tremblay
- Département de Biochimie, Microbiologie et de Bio-informatique, Faculté des Sciences et de Génie, Université Laval
- Deborah Jacobs-Sera
- Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh
- Mandana Sassanfar
- Department of Biology, Massachusetts Institute of Technology
- Yan-Jiun Lee
- Research Department, New England Biolabs
- Peter Weigele
- Research Department, New England Biolabs
- Sylvain Moineau
- Département de Biochimie, Microbiologie et de Bio-informatique, Faculté des Sciences et de Génie, Université Laval
- Graham F. Hatfull
- Pittsburgh Bacteriophage Institute and Department of Biological Sciences, University of Pittsburgh
- Peter C. Dedon
- Singapore-MIT Alliance for Research and Technology, Antimicrobial Resistance Interdisciplinary Research Group, Campus for Research Excellence and Technological Enterprise
- Lars H. Hansen
- Department of Environmental Science, Aarhus University
- Valérie de Crécy-Lagard
- Department of Microbiology and Cell Science, University of Florida
- DOI
- https://doi.org/10.1038/s41467-019-13384-y
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 12
Abstract
Viral genomic DNA is often modified to evade the host bacterial restriction system. Here the authors identified 2′-deoxy-7-deazaguanine modifications on phage DNA by comparative genomics and experimental validation, showing their role in genome protection.
