Unveiling the Ro60-Ro52 complex

Laura R. Rodríguez; Jesus Vicente de Julián-Ortiz; Fernando Rubio de la Rúa; Augusto Juste-Dolz; Ángel Maquieira; Haydar Abdulhakim Mohammad-Salim; Sofiane Benmetir; Federico Vicente Pallardó; Pilar González-Cabo; David Gimenez-Romero

doi:10.17179/excli2024-7141

EXCLI Journal : Experimental and Clinical Sciences (Jun 2024)

Unveiling the Ro60-Ro52 complex

Laura R. Rodríguez,
Jesus Vicente de Julián-Ortiz,
Fernando Rubio de la Rúa,
Augusto Juste-Dolz,
Ángel Maquieira,
Haydar Abdulhakim Mohammad-Salim,
Sofiane Benmetir,
Federico Vicente Pallardó,
Pilar González-Cabo,
David Gimenez-Romero

Affiliations

Laura R. Rodríguez: ORCiD; Present address: Stem Cells, Aging and Neurodegeneration Group, Department of Experimental Medical Science, Faculty of Medicine, Lund Stem Cell Center, Lund University, 22184, Lund, Sweden
Jesus Vicente de Julián-Ortiz: ORCiD; Molecular Topology and Drug Design Research Unit, Department of Physical Chemistry, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Valencia, Spain
Fernando Rubio de la Rúa: ORCiD; Department of Physical Chemistry, Faculty of Chemistry, University of Valencia, C/ Doctor Moliner 50, 46100, Burjassot, Spain
Augusto Juste-Dolz: ORCiD; Center for Research and Innovation on Bioengineering (Ci2B), Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain
Ángel Maquieira: ORCiD; Departamento de Química, Polytechnic University of Valencia, Camino de Vera s/n 46022, Valencia, Spain; Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, Camino de Vera s/n, 46022 Valencia, Spain
Haydar Abdulhakim Mohammad-Salim: ORCiD; Molecular Topology and Drug Design Research Unit, Department of Physical Chemistry, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Valencia, Spain; Faculty of Science, Department of Chemistry, University of Zakho, Zakho, Duhok 42001, Kurdistan Region, Iraq
Sofiane Benmetir: ORCiD; Molecular Topology and Drug Design Research Unit, Department of Physical Chemistry, Faculty of Pharmacy, University of Valencia, Av. Vicent Andrés Estellés s/n, 46100 Valencia, Spain; Process and Environmental Engineering Laboratory (LIPE), Faculty of Chemistry, University of Science and Technology of Oran Mohamed BOUDIAF, P.O. Box 1503, El Mnaouer, 31000 Oran, Algeria
Federico Vicente Pallardó: ORCiD; Department of Physiology, Faculty of Medicine and Dentistry. University of Valencia-INCLIVA, 46010 Valencia, Spain; CIBER Rare Diseases (CIBERER), 46010 Valencia, Spain
Pilar González-Cabo: ORCiD; Department of Physiology, Faculty of Medicine and Dentistry. University of Valencia-INCLIVA, 46010 Valencia, Spain. E-mail: [email protected]
David Gimenez-Romero: ORCiD; Department of Physical Chemistry, Faculty of Chemistry, University of Valencia, C/ Doctor Moliner 50, 46100, Burjassot, Spain. E-mail: [email protected]

DOI: https://doi.org/10.17179/excli2024-7141
Journal volume & issue: Vol. 23
pp. 888 – 903

Abstract

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The coexistence within a subcellular complex of inter-cellular proteins Ro60, responsible for preserving ncRNA quality, and Ro52, involved in intracellular proteolysis, has been a subject of ongoing debate. Employing molecular docking in tandem with experimental methods like Quartz Crystal Microbalance with Dissipation (QCM-D), Proximity Ligation Assay (PLA), and Indirect Immunofluorescence (IIF), we reveal the presence of Ro60 associating with Ro52 within the cytoplasm. This result unveils the formation of a weak transient complex with a Ka ≈ (3.7 ± 0.3) x 106 M-1, where the toroid-shaped Ro60 structure interacts with the Ro52’s Fc receptor, aligning horizontally within the PRY-SPRY domains of the Ro52’s homodimer. The stability of this complex relies on the interaction between Ro52 chain A and specific Ro60 residues, such as K133, W177, or L185, vital in the Ro60-YRNA bond. These findings bridge the role of Ro60 in YRNA management with Ro52's function in intracellular proteolysis, emphasizing the potential impact of transient complexes on cellular pathways.

Published in EXCLI Journal : Experimental and Clinical Sciences

ISSN: 1611-2156 (Online)
Publisher: IfADo - Leibniz Research Centre for Working Environment and Human Factors, Dortmund
Country of publisher: Germany
LCC subjects: Medicine: Internal medicine: Neoplasms. Tumors. Oncology. Including cancer and carcinogens; Science: Biology (General)
Website: http://www.excli.de

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