Effect of Fibroblast Growth Factor (FGF) on Some Serum Oxidative Parameters in Hyperglycemic Rats

Abstract

Wound healing is a complex and dynamic process that includes multiple biological pathways and has some successive healing periods. Most growth factor is responsible for wound healing. Fibroblast growth factor has a positive effect on wound healing problems that can be caused by diabetes. In the present study, we aimed to investigate exogenous effect bFGF supplementation on serum TBARS, RSH and NOx levels in hyperglysemic rats. Experiments were performed on 30 male Wistar albino rats (weight range: 200-250 g ). Rats were hyperglycemic with STZ (ip, 60 mg/kg). The experimental groups were divided into untreated and bFGF-treated subgroups. bGF was applied locally to the dorsalateral wounds of rats (10 ng/ml). After these administrations, on the 3th and 7th days of wound healing, the animals were sacrificed. Serum TBARS, RSH and NOx levels were recorded spectrophotometrically. The results were expressed as mean ± Standard deviation and the mean differences were compared by Anova Variance Analysis (p lt;0,05). When compared with the treatment group, on the 7th day and the 3rd day, it was found that the serum TBARS levels increased statistically in hyperglycemic rats(p lt;0,05). Both in the 3rd day of the untreated and 3rd day of the rats treated with bFGF may significant decrease in the serum RSH levels. bFGF application was found both enhancing and reducing effects on oxidative stres. In subsequent studies, the effect of bFGF, which has positive effects on diabetic wound healing, on oxidative events can be investigated in detail using different doses and different treatment periods.

Keywords

• bfgf
• diabetic wound healing
• free radicals
• oxidative stress
• diabetes mellitus