NAD-seq for profiling the NAD+ capped transcriptome of Arabidopsis thaliana

Abstract

Summary: Eukaryotic RNAs can be modified with a non-canonical 5′ nicotinamide adenine dinucleotide (NAD+) cap. NAD-seq identifies transcriptome-wide NAD+ capped RNAs. NAD-seq takes advantage of click chemistry to allow the capture of NAD+ capped RNAs. Unlike other approaches, NAD-seq does not require DNA synthesis on beads, but this technique uses full NAD+ capped transcripts eluted from beads as the substrates for strand-specific RNA sequencing library preparation.For complete details on the use and execution of this protocol, please refer to Yu et al. (2021).

Keywords

• Genomics
• RNAseq
• Model Organisms
• Plant sciences
• Molecular Biology
• Gene Expression