E15.5 Mouse Embryo Micro-CT Using a Bruker Skyscan 1172 Micro-CT

Elena Astanina; Sara Petrillo; Tullio Genova; Federico  Mussano; Federico  Bussolino

doi:10.21769/BioProtoc.4662

Bio-Protocol (May 2023)

E15.5 Mouse Embryo Micro-CT Using a Bruker Skyscan 1172 Micro-CT

Elena Astanina,
Sara Petrillo,
Tullio Genova,
Federico Mussano,
Federico Bussolino

Affiliations

Elena Astanina: Department of Oncology, University of Torino, Torino, ItalyCandiolo Cancer Institute-IRCCS-FPO, Candiolo, Italy
Sara Petrillo: Department of Molecular Biotechnology and Health Sciences, Molecular Biotechnology Center, University of Torino, Torino, Italy
Tullio Genova: Department of Life Sciences and Systems Biology, University of Torino, Torino, Italy
Federico Mussano: CIR Dental School, Department of Surgical Sciences, University of Torino, Via Nizza 230, 10126, Torino, Italy
Federico Bussolino: Department of Oncology, University of Torino, Torino, ItalyCandiolo Cancer Institute-IRCCS-FPO, Candiolo, Italy

DOI: https://doi.org/10.21769/BioProtoc.4662
Journal volume & issue: Vol. 13, no. 9

Abstract

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X-ray computed microtomography (µCT) is a powerful tool to reveal the 3D structure of tissues and organs. Compared with the traditional sectioning, staining, and microscopy image acquisition, it allows a better understanding of the morphology and a precise morphometric analysis. Here, we describe a method for 3D visualization and morphometric analysis by µCT scanning of the embryonic heart of iodine-stained E15.5 mouse embryos.

Published in Bio-Protocol

ISSN: 2331-8325 (Online)
Publisher: Bio-protocol LLC
Country of publisher: United States
LCC subjects: Science: Biology (General)
Website: https://bio-protocol.org

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