Insights into SusCD-mediated glycan import by a prominent gut symbiont

Declan A. Gray; Joshua B. R. White; Abraham O. Oluwole; Parthasarathi Rath; Amy J. Glenwright; Adam Mazur; Michael Zahn; Arnaud Baslé; Carl Morland; Sasha L. Evans; Alan Cartmell; Carol V. Robinson; Sebastian Hiller; Neil A. Ranson; David N. Bolam; Bert van den Berg

doi:10.1038/s41467-020-20285-y

Nature Communications (Jan 2021)

Insights into SusCD-mediated glycan import by a prominent gut symbiont

Declan A. Gray,
Joshua B. R. White,
Abraham O. Oluwole,
Parthasarathi Rath,
Amy J. Glenwright,
Adam Mazur,
Michael Zahn,
Arnaud Baslé,
Carl Morland,
Sasha L. Evans,
Alan Cartmell,
Carol V. Robinson,
Sebastian Hiller,
Neil A. Ranson,
David N. Bolam,
Bert van den Berg

Affiliations

Declan A. Gray: Biosciences Institute, The Medical School, Newcastle University
Joshua B. R. White: Astbury Centre for Structural Molecular Biology, Faculty of Biological Sciences, University of Leeds
Abraham O. Oluwole: Physical and Theoretical Chemistry Laboratory, University of Oxford
Parthasarathi Rath: Biozentrum, University of Basel
Amy J. Glenwright: Biosciences Institute, The Medical School, Newcastle University
Adam Mazur: Biozentrum, University of Basel
Michael Zahn: Biozentrum, University of Basel
Arnaud Baslé: Biosciences Institute, The Medical School, Newcastle University
Carl Morland: Biosciences Institute, The Medical School, Newcastle University
Sasha L. Evans: Astbury Centre for Structural Molecular Biology, Faculty of Biological Sciences, University of Leeds
Alan Cartmell: Institute of Systems, Molecular and Integrative Biology, University of Liverpool
Carol V. Robinson: Physical and Theoretical Chemistry Laboratory, University of Oxford
Sebastian Hiller: Biozentrum, University of Basel
Neil A. Ranson: Astbury Centre for Structural Molecular Biology, Faculty of Biological Sciences, University of Leeds
David N. Bolam: Biosciences Institute, The Medical School, Newcastle University
Bert van den Berg: Biosciences Institute, The Medical School, Newcastle University

DOI: https://doi.org/10.1038/s41467-020-20285-y
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 14

Abstract

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In Bacteroidetes, SusCD complexes mediate uptake of large nutrients across the outer membrane. SusCD structures in the apo state and in complex with β2,6 fructo-oligosaccharides reveal several substrate molecules in the binding cavity and suggest details of the pedal bin mechanism employed in glycan import.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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