Nature Communications (Jun 2019)
Inhibition of CRISPR-Cas9 ribonucleoprotein complex assembly by anti-CRISPR AcrIIC2
- Annoj Thavalingam,
- Zhi Cheng,
- Bianca Garcia,
- Xue Huang,
- Megha Shah,
- Wei Sun,
- Min Wang,
- Lucas Harrington,
- Sungwon Hwang,
- Yurima Hidalgo-Reyes,
- Erik J. Sontheimer,
- Jennifer Doudna,
- Alan R. Davidson,
- Trevor F. Moraes,
- Yanli Wang,
- Karen L. Maxwell
Affiliations
- Annoj Thavalingam
- Department of Biochemistry, University of Toronto
- Zhi Cheng
- Key Laboratory of RNA Biology, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
- Bianca Garcia
- Department of Molecular Genetics, University of Toronto
- Xue Huang
- Key Laboratory of RNA Biology, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
- Megha Shah
- Department of Biochemistry, University of Toronto
- Wei Sun
- Key Laboratory of RNA Biology, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
- Min Wang
- Key Laboratory of RNA Biology, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
- Lucas Harrington
- Department of Molecular and Cell Biology, University of California, Berkeley
- Sungwon Hwang
- Department of Biochemistry, University of Toronto
- Yurima Hidalgo-Reyes
- Department of Molecular Genetics, University of Toronto
- Erik J. Sontheimer
- RNA Therapeutics Institute, University of Massachusetts Medical School
- Jennifer Doudna
- Department of Molecular and Cell Biology, University of California, Berkeley
- Alan R. Davidson
- Department of Biochemistry, University of Toronto
- Trevor F. Moraes
- Department of Biochemistry, University of Toronto
- Yanli Wang
- Key Laboratory of RNA Biology, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
- Karen L. Maxwell
- Department of Biochemistry, University of Toronto
- DOI
- https://doi.org/10.1038/s41467-019-10577-3
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 11
Abstract
Anti-CRISPR proteins offer the means of regulating CRISPR-Cas9 activity. Here the authors present the structure and biochemical characterisation of AcrIIC2 Nme alone and in complex with Cas9.
