UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution

Paul Jannis Zurek; Philipp Knyphausen; Katharina Neufeld; Ahir Pushpanath; Florian Hollfelder

doi:10.1038/s41467-020-19687-9

Nature Communications (Nov 2020)

UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution

Paul Jannis Zurek,
Philipp Knyphausen,
Katharina Neufeld,
Ahir Pushpanath,
Florian Hollfelder

Affiliations

Paul Jannis Zurek: Department of Biochemistry, University of Cambridge
Philipp Knyphausen: Department of Biochemistry, University of Cambridge
Katharina Neufeld: Department of Biochemistry, University of Cambridge
Ahir Pushpanath: Johnson Matthey Plc
Florian Hollfelder: Department of Biochemistry, University of Cambridge

DOI: https://doi.org/10.1038/s41467-020-19687-9
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 10

Abstract

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The success of protein evolution is dependent on the sequence context mutations are introduced into. Here the authors present UMIC-seq that allows consensus generation for closely related genes by using unique molecular identifiers linked to gene variants.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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