Peran protein membran luar 55 kDa Salmonella typhi isolat Jember sebagai protein hemaglutinin dan adhesin

Abstract

Salmonella typhi is an obligate pathogen that is usually found in clinical specimen from typhoid fever patients. The pathogenicmechanism of bacteria are not fully elucidated especially its potential activity of the outer membrane protein (OMP) as hemaglutininand adhesion molecule. After identification, bacterial isolate of outer membrane protein fraction 12,5% SDS-PAGE were used to isolateOMP followed by hemaglutinin test and invitro adhesion test. The study showed that the 55 kDa protein of S. typhi is a hemaglutininprotein that could agglutinate, Wistar mouse erythrocytes. The 55 kDa OMP is also an adhesion protein showed by its activity toadherence to Wistar mice’s enterocyt. The increased dose of 55 kDa OMP will decrease the amount of S. typhi bacteria adherenced to Wistar mices enterocyt. Outer membrane protein 55 kDa S. typhi as hemagglutinin and also adhesion protein.

Keywords

• Salmonella typhi
• OMP
• Adhesin
• Hemaglutinin