In Vitro Degradation of Gelatin/carboxymethylcellulose Scaffolds for Skin Tissue Regeneration

Abstract

The field of tissue engineering has grown in response to many medical needs for tissue replacement. For the skin replacement, there have to develop the different types of tissue engineered skin substitutes. The artificial scaffold have to be a biodegradable material with appropriate degradation rate for skin regeneration. In this study, gelatin blended with carboxymethylcellulose (CMC) scaffold was fabricated via freeze drying method. The various gelatin and CMC ratios were 100/0, 90/10, 80/20, 70/30 and 60/40, respectively. In vitro degradation of the scaffold was done by degradation in lysozyme in PBS buffer within desired time. The swelling ratio of the scaffolds was done by immersing in PBS buffer for 2 h at 37oC. The results revealed that the gelatin/CMC (80:20) scaffold showed the highest value of swelling ratio and have an appropriate degradation rate with completely degraded after 36 h.