Generation of a TLR7 homozygous knockout human induced pluripotent stem cell line using CRISPR/Cas9

Hyeong-jun Han; Hyang-Hee Seo; Hyo-Won Han; Jung-Hyun Kim

Stem Cell Research (Oct 2019)

Generation of a TLR7 homozygous knockout human induced pluripotent stem cell line using CRISPR/Cas9

Hyeong-jun Han,
Hyang-Hee Seo,
Hyo-Won Han,
Jung-Hyun Kim

Affiliations

Hyeong-jun Han: Division of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of Korea
Hyang-Hee Seo: Division of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of Korea
Hyo-Won Han: Division of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of Korea
Jung-Hyun Kim: Corresponding author.; Division of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of Korea

Journal volume & issue: Vol. 40

Abstract

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Toll Like Receptor (TLR) family plays an important role in the activation of innate immunity against pathogens. TLR7 mediates the recognition of single-stranded RNA viruses, such as human immunodeficiency virus, hepatitis C virus, and influenza virus in endosomes. Here, we generated a TLR7 homozygous knockout human induced pluripotent cell (hiPSC) line, hiPSC-TLR7KO-A59, using the CRISPR/Cas9 genome editing method. The hiPSC-TLR7KO-A59 line maintains normal morphology, pluripotency, and differentiation capacity into three germ layers.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal