Scientific Reports (Nov 2023)

AePUb promoter length modulates gene expression in Aedes aegypti

  • Michelle A. E. Anderson,
  • Philip T. Leftwich,
  • Ray Wilson,
  • Leonela Z. Carabajal Paladino,
  • Sanjay Basu,
  • Sara Rooney,
  • Zach N. Adelman,
  • Luke Alphey

DOI
https://doi.org/10.1038/s41598-023-47777-3
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 7

Abstract

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Abstract Molecular tools for modulating transgene expression in Aedes aegypti are few. Here we demonstrate that adjustments to the AePUb promoter length can alter expression levels of two reporter proteins in Ae. aegypti cell culture and in mosquitoes. This provides a simple means for increasing or decreasing expression of a gene of interest and easy translation from cells to whole insects.