Phase separation of Polo-like kinase 4 by autoactivation and clustering drives centriole biogenesis

Abstract

Regulation of centriole duplication is essential for normal chromosome segregation and the maintenance of genomic stability, and Polo-like kinase 4 (Plk4) is a known regulator with unclear mechanisms. Here the authors show that Plk4 undergoes phase separation into an assembling body through autophosphorylation which drives centriole biogenesis.