Monolayer surface chemistry enables 2-colour single molecule localisation microscopy of adhesive ligands and adhesion proteins

Xun Lu; Philip R. Nicovich; Manchen Zhao; Daniel J. Nieves; Mahdie Mollazade; S. R. C. Vivekchand; Katharina Gaus; J. Justin Gooding

doi:10.1038/s41467-018-05837-7

Nature Communications (Aug 2018)

Monolayer surface chemistry enables 2-colour single molecule localisation microscopy of adhesive ligands and adhesion proteins

Xun Lu,
Philip R. Nicovich,
Manchen Zhao,
Daniel J. Nieves,
Mahdie Mollazade,
S. R. C. Vivekchand,
Katharina Gaus,
J. Justin Gooding

Affiliations

Xun Lu: School of Chemistry, Australian Centre for NanoMedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of New South Wales
Philip R. Nicovich: EMBL Australia Node in Single Molecule Science, School of Medical Sciences and the ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales
Manchen Zhao: School of Chemistry, Australian Centre for NanoMedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of New South Wales
Daniel J. Nieves: EMBL Australia Node in Single Molecule Science, School of Medical Sciences and the ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales
Mahdie Mollazade: EMBL Australia Node in Single Molecule Science, School of Medical Sciences and the ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales
S. R. C. Vivekchand: School of Chemistry, Australian Centre for NanoMedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of New South Wales
Katharina Gaus: EMBL Australia Node in Single Molecule Science, School of Medical Sciences and the ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales
J. Justin Gooding: School of Chemistry, Australian Centre for NanoMedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of New South Wales

DOI: https://doi.org/10.1038/s41467-018-05837-7
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 10

Abstract

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To date, the precise localisation of ligands and adhesion proteins are determined in two parallel characterization setups. Here, the authors report a self-assembled monolayer chemistry for indium tin oxide surfaces allowing single molecule localisation microscopy (SMLM) imaging of ligands and adhesion proteins in a single experiment.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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