Silencing SF3B1 promotes apoptosis and inhibits proliferation and invasion of human lung cancer cell line A549

Abstract

Objective To explore the effect of splicing factor 3B subunit (SF3B1) on apoptosis, proliferation and invasion of human lung cancer cells. Methods Non-small cell lung cancer(NSCLC) patients in the General Hospital of Western Theater Command PLA from June 2017 to June 2020 were selected as the research objects to detect the level of SF3B1 in cancer and adjacent tissues, and to analyze the relationship between SF3B1 and the pathological characteristics, survival and prognosis of patients. In addition, A549 cells were cultured and divided into control group, transfected si-NC group and si-SF3B1 group. Cell apoptosis was detected by flow cytometry, cell proliferation was detected by CCK-8, cell invasion was detected by Transwell, and the expression of Ras/Raf/ERK signal protein was detected by Western blot. Results The level of SF3B1 mRNA in cancer tissues was significantly higher than that in adjacent tissues(P＜0.05), and its level in A549 cells was significantly higher than that in normal lung epithelial cell line BEAS-2B(P＜0.05). The high level of SF3B1 was related to lymph node metastasis, tumor size and differentiation(P＜0.05). The median overall survival(OS) and progression free survival(PFS) of patients with low expression of SF3B1 were significantly higher than those with high expression of SF3B1(P＜0.05). After silencing SF3B1, the apoptosis of A549 cells was significantly increased but the proliferation and invasion were significantly decreased. The silence of SF3B1 suppressed the expression of Ras, Raf and p-ERK in A549 cells. Conclusions SF3B1 is highly expressed in NSCLC. Silencing SF3B1 can promote lung cancer cell apoptosis, inhibit cell proliferation and invasion.

Keywords

• non-small cell lung cancer|splicing factor 3b subunit 1|ras|raf|erk