Scientific Reports (Sep 2017)

CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs

  • Jun Wu,
  • Marcela Vilarino,
  • Keiichiro Suzuki,
  • Daiji Okamura,
  • Yanina Soledad Bogliotti,
  • Insung Park,
  • Joan Rowe,
  • Bret McNabb,
  • Pablo Juan Ross,
  • Juan Carlos Izpisua Belmonte

DOI
https://doi.org/10.1038/s41598-017-08596-5
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 6

Abstract

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Abstract Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs.