Magneto-nanosensor platform for probing low-affinity protein–protein interactions and identification of a low-affinity PD-L1/PD-L2 interaction

Jung-Rok Lee; Daniel J. B. Bechstein; Chin Chun Ooi; Ashka Patel; Richard S. Gaster; Elaine Ng; Lino C. Gonzalez; Shan X. Wang

doi:10.1038/ncomms12220

Nature Communications (Jul 2016)

Magneto-nanosensor platform for probing low-affinity protein–protein interactions and identification of a low-affinity PD-L1/PD-L2 interaction

Jung-Rok Lee,
Daniel J. B. Bechstein,
Chin Chun Ooi,
Ashka Patel,
Richard S. Gaster,
Elaine Ng,
Lino C. Gonzalez,
Shan X. Wang

Affiliations

Jung-Rok Lee: Department of Mechanical Engineering, Stanford University
Daniel J. B. Bechstein: Department of Mechanical Engineering, Stanford University
Chin Chun Ooi: Department of Chemical Engineering, Stanford University
Ashka Patel: Department of Proteomics and Biological Resources, Genentech
Richard S. Gaster: Department of Bioengineering, Stanford University
Elaine Ng: Department of Bioengineering, Stanford University
Lino C. Gonzalez: Department of Proteomics and Biological Resources, Genentech
Shan X. Wang: Department of Materials Science and Engineering, Stanford University

DOI: https://doi.org/10.1038/ncomms12220
Journal volume & issue: Vol. 7, no. 1
pp. 1 – 9

Abstract

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Measurement of low-affinity protein–protein interactions is challenging; surface plasmon resonance requires high concentrations of reagents. Here the authors combine magneto-nanosensors with microfluidic chips and protein-conjugated magnetic nanoparticles to discover a low-affinity interaction between T-cell inhibitory receptors PD-L1 and PD-L2.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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