Determination of eight kinds of insecticides in dairy products by dispersed solid phase extraction-ultra performance liquid chromatography-tandem mass spectrometry

Abstract

Objective: To detect eight kinds of insecticides in dairy products （pure milk, fermented milk, milk powder） rapidly. Methods: A high performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS） method based on dispersive solid phase extraction pre-treatment technology was established. The samples were extracted with acetonitrile-acetone solution （Vacetonitrile∶Vacetone=80∶20） in ultrasonic way, and the extraction solution was purified by Florisil, primary secondary amine and anhydrous MgSO4. After purification, the samples were analyzed by HPLC-MS/MS qualitatively and quantitatively in multi reaction monitoring （MRM） mode. Results: The correlation coefficient （r） of fenamiphos, imidacloprid, spinosad, dinotefuran, emamectin benzoate, methomyl, thiacloprid and oxamyl in the linear range of 2.00~100 μg/L was greater than 0.99, the limits of detection were 0.010~0.30 μg/kg, the recoveries of method were 82.6%~97.5%, and the relative standard deviations （RSDs） were 1.2%~4.6% （n=6）. Conclusion: The method is simple, rapid, sensitive and reproducible. It is suitable for the rapid screening of insecticides in dairy products.

Keywords

• dispersed solid phase extraction
• uplc-ms/ms
• dairy products
• insecticides