A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates

Andrey A. Petropavlovskiy; Michael G. Tauro; Patrick Lajoie; Martin L. Duennwald

STAR Protocols (Dec 2020)

A Quantitative Imaging-Based Protocol for Yeast Growth and Survival on Agar Plates

Andrey A. Petropavlovskiy,
Michael G. Tauro,
Patrick Lajoie,
Martin L. Duennwald

Affiliations

Andrey A. Petropavlovskiy: Department of Biology, The University of Western Ontario, London, ON N6A 3K7, Canada; Corresponding author
Michael G. Tauro: Department of Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, ON N6A 3K7, Canada
Patrick Lajoie: Department of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, ON N6A 3K7, Canada
Martin L. Duennwald: Department of Pathology and Laboratory Medicine, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, ON N6A 3K7, Canada; Department of Biology, The University of Western Ontario, London, ON N6A 3K7, Canada; Department of Anatomy and Cell Biology, Schulich School of Medicine and Dentistry, The University of Western Ontario, London, ON N6A 3K7, Canada; Corresponding author

Journal volume & issue: Vol. 1, no. 3
p. 100182

Abstract

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Summary: We present a detailed protocol that describes the evaluation of the growth and survival of yeast cells by quantitatively analyzing spotting assays. This simple method reproducibly detects and quantifies subtle differences in growth by measuring the density of cells within a single spot of defined size on an image of a spotting assay. Our protocol is tailored specifically for low-throughput applications, can be easily adapted for specific experimental conditions, and is accessible to yeast experts and non-experts alike.For an example of the execution of this protocol, please refer to DiGregorio et al. (Di Gregorio et al., 2020).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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Abstract

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