Frontiers in Chemistry (Dec 2022)

Solvent modulation in peptide sub-microfibers obtained by solution blow spinning

  • Ana Margarida Gonçalves Carvalho Dias,
  • Ana Margarida Gonçalves Carvalho Dias,
  • Cícero Cena,
  • Viviane Lutz-Bueno,
  • Viviane Lutz-Bueno,
  • Raffaele Mezzenga,
  • Ana Marques,
  • Ana Marques,
  • Isabel Ferreira,
  • Ana Cecília Afonso Roque,
  • Ana Cecília Afonso Roque

DOI
https://doi.org/10.3389/fchem.2022.1054347
Journal volume & issue
Vol. 10

Abstract

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Peptides possess high chemical diversity at the amino acid sequence level, which further translates into versatile functions. Peptides with self-assembling properties can be processed into diverse formats giving rise to bio-based materials. Peptide-based spun fibers are an interesting format due to high surface-area and versatility, though the field is still in its infancy due to the challenges in applying the synthetic polymer spinning processes to protein fibers to peptides. In this work we show the use of solution blow-spinning to produce peptide fibers. Peptide fiber formation was assisted by the polymer poly (vinyl pyrrolidone) (PVP) in two solvent conditions. Peptide miscibility and further self-assembling propensity in the solvents played a major role in fiber formation. When employing acetic acid as solvent, peptide fibers (0.5 μm) are formed around PVP fibers (0.75 μm), whereas in isopropanol only one type of fibers are formed, consisting of mixed peptide and PVP (1 μm). This report highlights solvent modulation as a mean to obtain different peptide sub-microfibers via a single injection nozzle in solution blow spinning. We anticipate this strategy to be applied to other small peptides with self-assembly propensity to obtain multi-functional proteinaceous fibers.

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