Influence of different cavity disinfectants on composite adhesion to dentin of primary teeth: An in vitro study

Ana Coelho; Luís Vilhena; Mariana Cordeiro; Inês Amaro; Anabela Paula; Carlos Miguel Marto; Cristina Cardoso Silva; Manuel Marques Ferreira; Eunice Carrilho; Amílcar Ramalho

Heliyon (Dec 2024)

Influence of different cavity disinfectants on composite adhesion to dentin of primary teeth: An in vitro study

Ana Coelho,
Luís Vilhena,
Mariana Cordeiro,
Inês Amaro,
Anabela Paula,
Carlos Miguel Marto,
Cristina Cardoso Silva,
Manuel Marques Ferreira,
Eunice Carrilho,
Amílcar Ramalho

Affiliations

Ana Coelho: Institute of Integrated Clinical Practice, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, Portugal; Area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, 3000-548 Coimbra, Portugal; Clinical Academic Center of Coimbra (CACC), 3004-561 Coimbra, Portugal; Department of Mechanical Engineering, Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), University of Coimbra, 3030-788 Coimbra, Portugal
Luís Vilhena: Department of Mechanical Engineering, Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), University of Coimbra, 3030-788 Coimbra, Portugal
Mariana Cordeiro: Institute of Integrated Clinical Practice, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, Portugal
Inês Amaro: Institute of Integrated Clinical Practice, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, Portugal; Area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, 3000-548 Coimbra, Portugal; Clinical Academic Center of Coimbra (CACC), 3004-561 Coimbra, Portugal; Department of Mechanical Engineering, Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), University of Coimbra, 3030-788 Coimbra, Portugal
Anabela Paula: Institute of Integrated Clinical Practice, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, Portugal; Area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, 3000-548 Coimbra, Portugal; Clinical Academic Center of Coimbra (CACC), 3004-561 Coimbra, Portugal; Department of Mechanical Engineering, Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), University of Coimbra, 3030-788 Coimbra, Portugal
Carlos Miguel Marto: Area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, 3000-548 Coimbra, Portugal; Clinical Academic Center of Coimbra (CACC), 3004-561 Coimbra, Portugal; Department of Mechanical Engineering, Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), University of Coimbra, 3030-788 Coimbra, Portugal; Institute of Biophysics, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal; Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, 3000-548 Coimbra, Portugal; Corresponding author. Area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, Portugal.
Cristina Cardoso Silva: Faculty of Health Sciences, University Fernando Pessoa, 4200-551 Porto, Portugal; FP – I3ID, FP – BHS, Faculty of Health Sciences, University Fernando Pessoa, 4200-551 Porto, Portugal
Manuel Marques Ferreira: Area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, 3000-548 Coimbra, Portugal; Clinical Academic Center of Coimbra (CACC), 3004-561 Coimbra, Portugal; Department of Mechanical Engineering, Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), University of Coimbra, 3030-788 Coimbra, Portugal; Institute of Endodontics, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, Portugal
Eunice Carrilho: Institute of Integrated Clinical Practice, Faculty of Medicine, University of Coimbra, 3000-075 Coimbra, Portugal; Area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, Coimbra Institute for Clinical and Biomedical Research (iCBR), University of Coimbra, 3000-548 Coimbra, Portugal; Clinical Academic Center of Coimbra (CACC), 3004-561 Coimbra, Portugal; Department of Mechanical Engineering, Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), University of Coimbra, 3030-788 Coimbra, Portugal
Amílcar Ramalho: Department of Mechanical Engineering, Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), University of Coimbra, 3030-788 Coimbra, Portugal

Journal volume & issue: Vol. 10, no. 23
p. e40263

Abstract

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Restorative treatment is the most common approach to treating dental caries. However, after cavity preparation, some microorganisms may still persist in the substrate, suggesting the use of cavity disinfectants. Nevertheless, their effect on adhesion to composite resins is not yet fully understood, especially in primary teeth. The study aimed to assess the impact of five different cavity disinfectants on dentin adhesion in primary teeth. A total of 60 primary molars were uniformly flattened at their occlusal thirds and randomly allocated into six groups (n = 10 each): Control; Glutaraldehyde; Chlorhexidine; EDTA; Ethanol; Aloe vera. All disinfectants were actively applied, rinsed, and air-dried. The adhesion procedure was carried out according to the manufacturer's instructions, and the restoration was positioned using a mold. Shear bond strength was evaluated. Data were statistically analyzed (One-way ANOVA with Post-hoc Tukey test), with the level of significance set at 5 %. Glutaraldehyde (14.59 ± 3.89 MPa), Chlorhexidine (11.24 ± 2.25 MPa), and EDTA (11.04 ± 2.95 MPa) did not impair the shear bond strength when compared to the Control group (14.95 ± 2.75 MPa). Ethanol and Aloe vera application significantly lowered SBS. The results suggest that Glutaraldehyde, Chlorhexidine, and EDTA can be used as cavity disinfectants. Nevertheless, further in vitro and clinical research is required.

Published in Heliyon

ISSN: 2405-8440 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Science (General); Social Sciences: Social sciences (General)
Website: https://www.cell.com/heliyon/home

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