Molecular Detection of Trichomonas tenax Using Real-Time Gingivitis Patients in Babylon

Abstract

Background:Studies indicate that Trichomonas tenax is a significant factor in the development of gingivitis and periodontitis in the gingival region. This organism is an opportunist that can thrive in the environment created by periodontal disease. Objectives:The aim of this study is to detect T. tenax using real-time quantitative polymerase chain reaction (RT-qPCR) technique in plaque-induced gingivitis (PIG) patients in Babylon. Materials and Methods:This study involved 50 healthy participants (control group) and 50 patients with PIG. Saliva samples were collected from each patient and transported to the Alharithia and Baghdad laboratories of the Advancement Scientific Office Learning Center for molecular testing. Results:T. tenax infection was identified using RT-qPCR and melting curve analysis in 16% of the gingivitis patients (PIG) exposed to plaque. Furthermore, a statistically significant correlation was observed between patient age groups and the presence of PIG disease, as indicated by the t test (27.568, P = 0.000), and between sex, as determined by the t test (8.573, P = 0.000). Conclusion:This study underscores the higher prevalence of T. tenax in patients with PIG disease. Furthermore, it highlights the correlation between these presence of these parasites and the age and sex of PIG disease patients.

Keywords

• pig disease
• rt-qpcr
• trichomonas tenax