Synthesis and activity in blood clotting systems of unsaturated racemic phosphatidyl serines

Abstract

The synthesis of racemic 1,2-dioleoyl and 1-ole-oyl-2-stearoyl glycerol-3-phosphoryl serine was achieved by the phosphorylation of unsaturated diglycerides with phosphorus oxychloride, and combination with the phthalimidomethyl ester of carbo-t-butoxy-dl-serine.The synthetic phosphatidyl serines were tested in blood clotting systems in vitro. They have activity qualitatively similar to that of naturally occurring phosphatidyl serines: anticoagulant activity when well solubilized and weak to moderate thromboplastic activity in simple suspension.