INHIBITION OF CELLULAR INVASION AND INDUCTION OF ANOIKIS IN MOUSE MELANOMA CELLS BY AN ANTI-INFLAMMATORY AGENT DTCM-GLUTARIMIDE

Abstract

Previously, we designed DTCM-glutarimide as a novel anti-inflammatory agent inhibiting macrophage activation. It also showed anti-inflammatory activity in vivo. In the present research, we evaluated the anti-metastatic activity of this agent using mouse melanoma B16-F10 cells. DTCM-glutarimide inhibited the cellular invasion in the Matrigel chamber assay, lowering matrix metalloproteinase 9 (MMP9) expression. It also induced anoikis rather than apoptosis in polyHEMA-coated plates. It increased Bax and decreased Bcl-XL expressions, and activated p53 lowereing the MDM2 expression. Thus, DTCM-glutarimide was found to inhibit cellular invasion and to induce anoikis in B16-F10 cells. It is non-toxic, and easily prepared, therefore, is considered to be a new candidate of anti-metastasis agent.

Keywords

• dtcm-глутаримид
• инвазия
• аноикоз
• bax
• bcl-xl
• p53