The Codelivery of siRNA and QDs by pH-Responsive Micelle for Hepatoma Cancer Cells

Zhonglin Cao; Zhonglin Cao; Huiyu Xiao; Li Li; Maixian Liu; Maixian Liu; Guimiao Lin; Peng Zhai; Ken-Tye Yong; Xiaomei Wang; Gaixia Xu

doi:10.3389/fphar.2019.01194

Frontiers in Pharmacology (Oct 2019)

The Codelivery of siRNA and QDs by pH-Responsive Micelle for Hepatoma Cancer Cells

Zhonglin Cao,
Zhonglin Cao,
Huiyu Xiao,
Li Li,
Maixian Liu,
Maixian Liu,
Guimiao Lin,
Peng Zhai,
Ken-Tye Yong,
Xiaomei Wang,
Gaixia Xu

Affiliations

Zhonglin Cao: Key Laboratory of Optoelectronics Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, China
Zhonglin Cao: Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, China
Huiyu Xiao: Key Laboratory of Optoelectronics Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, China
Li Li: Department of Physiology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center, Shenzhen, China
Maixian Liu: Key Laboratory of Optoelectronics Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, China
Maixian Liu: Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, China
Guimiao Lin: Department of Physiology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center, Shenzhen, China
Peng Zhai: Department of Physiology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center, Shenzhen, China
Ken-Tye Yong: School of Electrical and Electronic Engineering, Nanyang Technological University, Singapore, Singapore
Xiaomei Wang: Department of Physiology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center, Shenzhen, China
Gaixia Xu: Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, China

DOI: https://doi.org/10.3389/fphar.2019.01194
Journal volume & issue: Vol. 10

Abstract

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Recently, RNA interfering (RNAi) has become a promising approach for cancer therapy. However, the application of RNAi for clinics is still hindered due to the lack of safe and efficient carriers. In this study, a pH-responsive micelle based on polycaprolactone-block–poly 2-(dimethylamino)ethyl methacrylate (PCL-PDEM) cationic copolymer was developed to carry short interfering RNA (siRNA) for silencing interleukin 8 (IL-8) gene in hepatoma cancer cells. The transfection efficiency of the PCL-PDEM-siRNA/quantum dots (QDs) nanoplex has reached about 70%, and the expression level of IL-8 decreased about 63%. Furthermore, the codelivery of QDs and siRNA has been realized, which is beneficial to visualize the process of siRNA delivery. No considerable cytotoxicity from the nanoparticles has been observed, indicating that our responsive cationic micelle is potential in clinical trial for hepatoma cancer therapy.

Published in Frontiers in Pharmacology

ISSN: 1663-9812 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://journal.frontiersin.org/journals/pharmacology

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