Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul (Jun 2006)
Effect of Betadine on wound healing in rat
Abstract
BACKGROUND & OBJECTIVE: Betadine is a disinfectant routinely used in all of the Iranian health centers and many other countries in the world but its effect on wound healing is controversial. The aim of this study was to investigate the effect of betadine on wound healing in rat.METHODS: In this interventional study, two groups of rats (n=10 each) were randomized as betadine and control groups. Under nesdonal anesthesia, a full thickness skin in an area of 2 cm2 was excised on dorsal neck of rats and immediately betadine was applied in betadine group. Control group received no treatment. Wound area, percentage of recovery and body weight were measured on postoperative days 1, 4, 7 and 10. Number of fibroblasts, collagen fibers, new vessels, lymphocytes, macrophages, neutrophils and thickness of epiderm were determined in three groups by biopsy on day 10.FINDINGS: The results showed the percentage of recovery was not significantly different in the 4th, 7th and 10th days. Revascularisation, fibroblasts, lymphocytes, neutrophils, epidermal depth and collagen fibers were not significantly different between two groups. Macrophages in the betadine group were significantly higher than control group (p<0.01).CONCLUSON: The results propose that betadine not only does not speed up wound healing but also delays the inflammatory phase.
