PHOSPHAN Microplate Technology-Based Microarray For Detection of IgG Antibodies against West Nile, Crimean Congo Hemorrhagic Fever and Tick-Borne Encephalitis Viruses

Abstract

Demonstrated was the possibility to use PHOSPHAN microplate technology to examine human sera and detect simultaneously specific IgG antibodies to three arboviruses, including the West Nile virus (WNV), Crimean Congo Hemorrhagic Fever virus (CCHFV), and Tick-borne encephalitis virus (TBEV). Both sensitivity and specificity of microarray immunoassay approach were similar to those of ELISA tests (when serum specimens were investigated separately). Using the criterion of 2-fold or stronger reaction of the examined serum specimen with homologous antigen than with heterologous one, we succeeded in differentiation of group specific anti WNV and anti TBEV IgG antibodies in about 60% of cross reactive sera. From the economical standpoint, the PHOSPAN technology may have advantages as compared with currently used ELISAs due to miniaturization of immunoassay format and ability to multiplex.

Keywords

• west nile virus
• crimean-congo hemorrhagic fever virus
• tick-borne encephalitis virus
• antigen microarray
• phosphorescence assay
• phosphan
• serodiagnosis