Platelets (Nov 2022)

Comparison of the effects of platelet concentrates produced by high and low-speed centrifugation protocols on the healing of critical-size defects in rat calvaria: a microtomographic and histomorphometric study

  • Lucia Moitrel Pequeno da Silva,
  • Débora de Souza Ferreira Sávio,
  • Felipe Correa de Ávila,
  • Raphael Martini Vicente,
  • Gabriel Guerra David Reis,
  • Ricardo Junior Denardi,
  • Natacha Malu Miranda da Costa,
  • Pedro Henrique Felix Silva,
  • Carlos Fernando de Almeida Barros Mourão,
  • Richard J. Miron,
  • Michel Reis Messora

DOI
https://doi.org/10.1080/09537104.2022.2071851
Journal volume & issue
Vol. 33, no. 8
pp. 1175 – 1184

Abstract

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The current study evaluated the healing of critical-size defects (CSD) created in rat calvaria treated with platelet concentrates produced by high-speed (Leukocyte- and Platelet-Rich Fibrin - L-PRF) and low-speed (Advanced Platelet-Rich Fibrin - A-PRF) protocols of centrifugation. Twenty-four rats were distributed into three groups: Control, L-PRF, and A-PRF. Five mm diameter CSD were created on the animals’ calvaria. The defects of the L-PRF and A-PRF groups were filled with 0.01 ml of L-PRF and A-PRF, respectively. The control group defects were filled with a blood clot only. All animals were euthanized on the 35th postoperative day. Histomorphometric and microtomographic analyses were then performed. The L-PRF and A-PRF groups had significantly higher bone volume and neoformed bone area than those of the control group and lowered bone porosity values (p < .05). No significant differences were observed between A-PRF and L-PRF groups for the analyzed parameters. Therefore, it can be concluded that i) L-PRF and A-PRF potentiated the healing of CSD in rat calvaria; ii) high and low-speed centrifugation protocols did not produce PRF matrices with different biological impacts on the amount of bone neoformation.

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