CGF-HLC-I repaired the bone defect repair of the rabbits mandible through tight junction pathway

Yalin Mao; Yalin Mao; Miaoling Hu; Miaoling Hu; Li Chen; Li Chen; Xiao Chen; Xiao Chen; Maohua Liu; Maohua Liu; Menglian Zhang; Menglian Zhang; Minhai Nie; Minhai Nie; Xuqian Liu; Xuqian Liu

doi:10.3389/fbioe.2022.976499

Frontiers in Bioengineering and Biotechnology (Sep 2022)

CGF-HLC-I repaired the bone defect repair of the rabbits mandible through tight junction pathway

Yalin Mao,
Yalin Mao,
Miaoling Hu,
Miaoling Hu,
Li Chen,
Li Chen,
Xiao Chen,
Xiao Chen,
Maohua Liu,
Maohua Liu,
Menglian Zhang,
Menglian Zhang,
Minhai Nie,
Minhai Nie,
Xuqian Liu,
Xuqian Liu

Affiliations

Yalin Mao: Department of Periodontics and Oral Mucosal Diseases, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou, Sichuan, China
Yalin Mao: Luzhou Key Laboratory of Oral and Maxillofacial Reconstruction and Regeneration, Southwest Medical University, Luzhou, China
Miaoling Hu: Department of Periodontics and Oral Mucosal Diseases, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou, Sichuan, China
Miaoling Hu: Luzhou Key Laboratory of Oral and Maxillofacial Reconstruction and Regeneration, Southwest Medical University, Luzhou, China
Li Chen: Department of Periodontics and Oral Mucosal Diseases, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou, Sichuan, China
Li Chen: Luzhou Key Laboratory of Oral and Maxillofacial Reconstruction and Regeneration, Southwest Medical University, Luzhou, China
Xiao Chen: Department of Stomatology Technology, School of Medical Technology, Sichuan College of Traditional Medcine, Mianyang, China
Xiao Chen: Department of Orthodontics, Mianyang Stomatological Hospital, Mianyang, China
Maohua Liu: Department of Periodontics and Oral Mucosal Diseases, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou, Sichuan, China
Maohua Liu: Luzhou Key Laboratory of Oral and Maxillofacial Reconstruction and Regeneration, Southwest Medical University, Luzhou, China
Menglian Zhang: Department of Periodontics and Oral Mucosal Diseases, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou, Sichuan, China
Menglian Zhang: Luzhou Key Laboratory of Oral and Maxillofacial Reconstruction and Regeneration, Southwest Medical University, Luzhou, China
Minhai Nie: Department of Periodontics and Oral Mucosal Diseases, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou, Sichuan, China
Minhai Nie: Luzhou Key Laboratory of Oral and Maxillofacial Reconstruction and Regeneration, Southwest Medical University, Luzhou, China
Xuqian Liu: Department of Periodontics and Oral Mucosal Diseases, The Affiliated Stomatological Hospital of Southwest Medical University, Luzhou, Sichuan, China
Xuqian Liu: Luzhou Key Laboratory of Oral and Maxillofacial Reconstruction and Regeneration, Southwest Medical University, Luzhou, China

DOI: https://doi.org/10.3389/fbioe.2022.976499
Journal volume & issue: Vol. 10

Abstract

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Background: The human-like collagen I (HLC-I) combined concentrated growth factors was used to construct CGF-HLC-I composite biomaterials to repair the critical bone defect disease model of rabbit mandible. This study aimed to research the repair mechanism of CGF-HLC-I/Bio-Oss in rabbit mandibular critical bone defect, to provide a new treatment direction for clinical bone defect repair.Methods: The optimal concentration of HLC-I (0.75%) was selected in this study. Nine New Zealand white rabbits were randomly divided into 3 groups, normal control group, Bio-Gide/Bio-Oss and CGF-0.75%HLC-I/Bio-Oss group (n = 3, each group). CGF-0.75%HLC-I/Bio-Oss and Bio-Gide/Bio-Oss were implanted into rabbit mandibles, then X-ray, Micro-CT, HE and Masson staining, immunohistochemical staining and biomechanical testing were performed with the bone continuity or maturity at 4, 8 and 12 weeks after surgery. The repair mechanism was studied by bioinformatics experiments.Results: As the material degraded, the rate of new bone formation in the CGF-0.75% HLC-I/Bio-Oss group was better than that the control group by micro-CT. The biomechanical test showed that the compressive strength and elastic modulus of the CGF-0.75%HLC-I/Bio-Oss group were higher than those of the control group. HE and Masson staining showed that the bone continuity or maturity of the CGF-0.75%HLC-I/Bio-Oss group was better than that of the control group. Immunohistochemical staining showed significantly higher bone morphogenetic protein 2 (BMP2) and Runt-related transcription factor 2 (RUNX2) in the CGF-0.75%HLC-I/Bio-Oss group than the control group at 8 and 12 W and the difference gradually decreased with time. There were 131 differentially expressed proteins (DEPs) in the Bio-Gide/Bio-Oss and CGF-0.75%HLC-I/Bio-Oss groups, containing 95 up-regulated proteins and 36 down-regulated proteins. KEGG database enrichment analysis showed actinin alpha 1 (ACTN1) and myosin heavy-Chain 9 (MYH9) are the main potential differential proteins related to osteogenesis, and they are enriched in the TJs pathway.Conclusion: CGF-0.75%HLC-I/Bio-Oss materials are good biomaterials for bone regeneration which have strong osteoinductive activity. CGF-0.75%HLC-I/Bio-Oss materials can promote new bone formation, providing new ideas for the application of bone tissue engineering scaffold materials in oral clinics.

Published in Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.frontiersin.org/bioengineering_and_biotechnology

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