Effect of Various Thawing Times and Temperatures on Frozen Semen Quality in Plastic Straws of Artificial Insemination of Bulls in Iraq

Abstract

The present study was designed to determine the best practical method of semen thawing which could be applied to frozen semen of bulls in straw produced by Artificial Insemination Center, Iraq. Frozen semen was thawed at 15°C for 30 sec, 40°C for 10 sec and 50°C for 5 sec. individual motility, live, and dead sperm cells was assessed using light microscope. Results revealed that number, motility, and live of sperm cells, were significantly higher at the 50°C for 5 sec than other thawing temperatures In conclusion, it is recommended to use a temperatures of 50°C for 5 sec and to dissolve the frozen semen because it gave the best quality semen after thawing and easy to utilize in field.

Keywords

• Frozen sperm
• Temperature
• Thawing time