Establishment of a chip digital PCR detection method for canine circovirus

Xiaoxiao Lei; Qiao Lv; Yan Qin; Wei Chen; Yanqing Hu; Chenchen Zhao; Xinyu Zhang; Haixin Huang; Yuying Li; Jingyi Lu; Tian Lan; Wenchao Sun; Min Zheng

Heliyon (May 2024)

Establishment of a chip digital PCR detection method for canine circovirus

Xiaoxiao Lei,
Qiao Lv,
Yan Qin,
Wei Chen,
Yanqing Hu,
Chenchen Zhao,
Xinyu Zhang,
Haixin Huang,
Yuying Li,
Jingyi Lu,
Tian Lan,
Wenchao Sun,
Min Zheng

Affiliations

Xiaoxiao Lei: Wenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, 325035, China
Qiao Lv: Wenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, 325035, China
Yan Qin: Wenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, 325035, China
Wei Chen: Wenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, 325035, China
Yanqing Hu: Wenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, 325035, China
Chenchen Zhao: Wenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, 325035, China
Xinyu Zhang: Wenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, 325035, China
Haixin Huang: Wenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, 325035, China
Yuying Li: Wenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, 325035, China
Jingyi Lu: Wenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, 325035, China
Tian Lan: Wenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, 325035, China; Corresponding author.
Wenchao Sun: Wenzhou Key Laboratory for Virology and Immunology, Institute of Virology, Wenzhou University, Wenzhou, 325035, China; Corresponding author.
Min Zheng: Guangxi Centre for Animal Disease Control and Prevention, Nanning, 530001, China; Corresponding author.

Journal volume & issue: Vol. 10, no. 9
p. e30859

Abstract

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Canine circovirus (CanineCV), which is a new mammalian circovirus first reported in the United States in 2012, mainly causes diarrhea and vomiting in dogs. As CanineCV evolves and new subtypes emerge, there is an urgent need for new detection technologies to improve the sensitivity and detection rates of viruses in complex scenarios. A chip digital PCR(cdPCR) assay was established for the detection of CanineCV in this study. The results showed good reproducibility, specificity and a linear relationship; the minimum detection limit of CanineCV by cdPCR was 6.62 copies/μL, which is 10 times more sensitive than quantitative real-time PCR (qPCR). The qPCR-positive detection rate was 1 %, while CanineCV cdPCR (2.1 %) exhibited a greater positive detection rate. Fifteen complete genomes were sequenced and subdivided into CanineCV-1 and CanineCV-3. In conclusion, we developed a rapid, reliable, and specific cdPCR method for screening and monitoring canine CV.

Published in Heliyon

ISSN: 2405-8440 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Science (General); Social Sciences: Social sciences (General)
Website: https://www.cell.com/heliyon/home

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