Fayixue Zazhi (Feb 2023)
Changes of 1，5-AG in Vitreous Humor of Rabbit Cadavers with Hyperglycemic Metabolism
Abstract
ObjectiveTo investigate the concentration and change characteristics of 1，5-anhydroglucitol （1，5-AG） in the vitreous humor of rabbit cadavers with hyperglycemic metabolism, and to explore the value of 1，5-AG in forensic pathology identification of death caused by hyperglycemic metabolism disorders.MethodsA diabetic hyperglycemic rabbit model was established by using alloxan. Eighteen rabbits with fasting glucose concentration ≥13.80 mmol/L （experimental group） and 18 healthy rabbits with fasting glucose concentration ≤6.10 mmol/L （control group） were selected. After death from air embolism. The blood samples were collected immediately, and vitreous humor samples were collected at 0 h, 12 h, 24 h and 36 h after death. The concentration of 1，5-AG in the blood and vitreous humor of rabbits was determined.ResultsThe blood glucose concentration in the experimental group was （25.10±3.14） mmol/L. At the time of death, there was no significant difference in the concentration of 1，5-AG in the blood [（0.94±0.20） μg/mL] and in the vitreous humor （0.99±0.05 μg/mL, P>0.05）. The concentration of 1，5-AG in the vitreous humor of the experimental group was lower than that of the corresponding control group at all time points （P0.05）. Correlation analysis showed that the concentration of 1,5-AG in blood was negatively correlated with blood glucose in both control group and experimental group （control group: r=-0.79, P<0.05; experimental group: r=-0.97, P<0.05）.ConclusionVitreous humor can replace blood as an effective test sample for 1,5-AG detection. The concentration of 1，5-AG in rabbit vitreous humor remains stable within 36 hours after death and is not affected by the change of postmortem interval. If the concentration of 1，5-AG decreases significantly, it indicates the existence of hyperglycemia in rabbits before death.
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