Analysis of the effectiveness of callusogenesis on media developed on the basis of the RZ medium

Abstract

There is still no consensus on the composition of nutrient media for various genotypes, and the improvement of their composition is still an urgent problem, the solution of which can significantly speed up and facilitate the breeding process, both in traditional and heterotic breeding. In this regard, the main goal of the study was to compile new variants of nutrient media and establish the relationship between changes in the components of the medium and the efficiency of callus formation. Previously, we showed the advantage of the RZ nutrient medium (Raina, Zapata. 1997) over the N nutrient medium (Nitsch, Nitsch 1969) for domestic varieties and hybrids, but to further increase the efficiency of callusogenesis, 5 variants based on the RZ nutrient medium were developed. The nutrient medium R1 was characterized by a reduced content of CaCl2·2H2O by almost three times, however, the results obtained on it were significantly better than on the initial nutrient medium RZ.