Blood-derived integration-free induced pluripotent stem cells (iPSCs) from one 53-years-old male donor with APOE-ε4/ε4 genotype

Xiaowei Ma; Jun Ma; Ruiyun Guo; Juan Du; Baofeng Feng; Xin Liu; Xiaofeng Du; Zhenhuan Ma; Huixian Cui

Stem Cell Research (Jul 2021)

Blood-derived integration-free induced pluripotent stem cells (iPSCs) from one 53-years-old male donor with APOE-ε4/ε4 genotype

Xiaowei Ma,
Jun Ma,
Ruiyun Guo,
Juan Du,
Baofeng Feng,
Xin Liu,
Xiaofeng Du,
Zhenhuan Ma,
Huixian Cui

Affiliations

Xiaowei Ma: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China; Department of Neurology, The First Hospital of Hebei Medical University, Hebei Province 050017, China
Jun Ma: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China; Human Anatomy Department, Hebei Medical University, Hebei Province 050017, China
Ruiyun Guo: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Juan Du: Human Anatomy Department, Hebei Medical University, Hebei Province 050017, China
Baofeng Feng: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Xin Liu: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Xiaofeng Du: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Zhenhuan Ma: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China
Huixian Cui: Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China; Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Province 050017, China; Human Anatomy Department, Hebei Medical University, Hebei Province 050017, China; Corresponding authors at: Hebei Research Center for Stem Cell Medical Translational Engineering, Hebei Medical University-National University of Ireland Galway Stem Cell Research Center, Hebei Medical University, Hebei Province 050017, China.

Journal volume & issue: Vol. 54
p. 102450

Abstract

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Apolipoprotein E ε4 allele (APOE4) is a minor allele of the APOE gene associated with a higher risk for Alzheimer's Disease (AD) and Vascular Dementia (VD). While lipid deposition and chronic inflammation in glia are the commonalities between atherosclerosis, VD, and AD. Hence, we presented an iPSC line of an AD male donor suffering from Cerebrovascular Atherosclerosis with APOE-ε4/ε4 alleles background. Furthermore, we differentiated the iPSCs into astrocyte to explore pathogenesis in APOE4 related dementia. The characterized iPSC line expressed typical pluripotency markers and showed differentiation potential and normal karyotype.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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