Overexpression of the Peach Transcription Factor Early Bud-Break 1 Leads to More Branches in Poplar

Xuehui Zhao; Xuehui Zhao; Xuehui Zhao; Binbin Wen; Binbin Wen; Binbin Wen; Chen Li; Chen Li; Chen Li; Qiuping Tan; Qiuping Tan; Qiuping Tan; Li Liu; Xiude Chen; Xiude Chen; Xiude Chen; Ling Li; Ling Li; Ling Li; Xiling Fu; Xiling Fu; Xiling Fu

doi:10.3389/fpls.2021.681283

Frontiers in Plant Science (Jun 2021)

Overexpression of the Peach Transcription Factor Early Bud-Break 1 Leads to More Branches in Poplar

Xuehui Zhao,
Xuehui Zhao,
Xuehui Zhao,
Binbin Wen,
Binbin Wen,
Binbin Wen,
Chen Li,
Chen Li,
Chen Li,
Qiuping Tan,
Qiuping Tan,
Qiuping Tan,
Li Liu,
Xiude Chen,
Xiude Chen,
Xiude Chen,
Ling Li,
Ling Li,
Ling Li,
Xiling Fu,
Xiling Fu,
Xiling Fu

Affiliations

Xuehui Zhao: College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
Xuehui Zhao: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
Xuehui Zhao: Shandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, China
Binbin Wen: College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
Binbin Wen: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
Binbin Wen: Shandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, China
Chen Li: College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
Chen Li: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
Chen Li: Shandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, China
Qiuping Tan: College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
Qiuping Tan: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
Qiuping Tan: Shandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, China
Li Liu: Shandong Academy of Grape, Shandong Academy of Agricultural Sciences, Jinan, China
Xiude Chen: College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
Xiude Chen: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
Xiude Chen: Shandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, China
Ling Li: College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
Ling Li: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
Ling Li: Shandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, China
Xiling Fu: College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, China
Xiling Fu: State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an, China
Xiling Fu: Shandong Collaborative Innovation Center for Fruit and Vegetable Production With High Quality and Efficiency, Tai’an, China

DOI: https://doi.org/10.3389/fpls.2021.681283
Journal volume & issue: Vol. 12

Abstract

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Shoot branching is an important adaptive trait that determines plant architecture. In a previous study, the Early bud-break 1 (EBB1) gene in peach (Prunus persica var. nectarina) cultivar Zhongyou 4 was transformed into poplar (Populus trichocarpa). PpEBB1-oe poplar showed a more branched phenotype. To understand the potential mechanisms underlying the EBB1-mediated branching, transcriptomic and proteomics analyses were used. The results showed that a large number of differentially expressed genes (DEGs)/differentially expressed proteins (DEPs) associated with light response, sugars, brassinosteroids (BR), and nitrogen metabolism were significantly enriched in PpEBB1-oe poplar. In addition, contents of sugars, BR, and amino acids were measured. Results showed that PpEBB1 significantly promoted the accumulation of fructose, glucose, sucrose, trehalose, and starch. Contents of brassinolide (BL), castasterone (CS), and 6-deoxocathasterone (6-deoxoCS) were all significantly changed with overexpressing PpEBB1. Various types of amino acids were measured and four of them were significantly improved in PpEBB1-oe poplar, including aspartic acid (Asp), arginine (Arg), cysteine (Cys), and tryptohpan (Trp). Taken together, shoot branching is a process controlled by a complex regulatory network, and PpEBB1 may play important roles in this process through the coordinating multiple metabolic pathways involved in shoot branching, including light response, phytohormones, sugars, and nitrogen.

Published in Frontiers in Plant Science

ISSN: 1664-462X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: https://www.frontiersin.org/journals/plant-science

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