Correlation between <i>Peptacetobacter hiranonis</i>, the <i>baiCD</i> Gene, and Secondary Bile Acids in Dogs

Abstract

Bile acid metabolism is a key pathway modulated by intestinal microbiota. Peptacetobacter (Clostridium) hiranonis has been described as the main species responsible for the conversion of primary into secondary fecal unconjugated bile acids (fUBA) in dogs. This multi-step biochemical pathway is encoded by the bile acid-inducible (bai) operon. We aimed to assess the correlation between P. hiranonis abundance, the abundance of one specific gene of the bai operon (baiCD), and secondary fUBA concentrations. In this retrospective study, 133 fecal samples were analyzed from 24 dogs. The abundances of P. hiranonis and baiCD were determined using qPCR. The concentration of fUBA was measured by gas chromatography–mass spectrometry. The baiCD abundance exhibited a strong positive correlation with secondary fUBA (ρ = 0.7377, 95% CI (0.6461, 0.8084), p P. hiranonis and secondary fUBA (ρ = 0.6658, 95% CI (0.5555, 0.7532), p 0.0001). Animals displaying conversion of fUBA and lacking P. hiranonis were not observed. These results suggest P. hiranonis is the main converter of primary to secondary bile acids in dogs.

Keywords

• fecal unconjugated bile acids
• canine
• <i>Clostridium hiranonis</i>
• primary bile acids
• conversion of bile acids
• bile acid metabolism