Aminoacids composition of muscular tissue of Coregonus lavaretus L. (the Kola Peninsula)

Abstract

The studies of the protein aminoacid composition and free aminoacids of muscle tissue of different ages of Coregonus lavaretus L. from Lakes Umbozero and Lovozero (the Kola Peninsula) have been presented. The research of aminoacid spectra in separate tissues allows to some extent to evaluate the features of protein metabolism and the physiological state of the organism. Aminoacid composition of proteins has been investigated by high-performance liquid chromatography. Fractional composition of the proteins' solubility has been determined by spectrophotometry. Significant differences in the ratio of bound and free aminoacids of muscle tissue of Coregonus lavaretus L. have been substantiated. It is established that the proteins of the muscle tissue of whitefish from Lake Lovozero have a higher total content of essential aminoacids unlike whitefish from Lake Umbozero. The content of essential aminoacid leucine in the proteins of muscle tissue of whitefish from Lovozero increases significantly, which is associated with an increase in the content of salt-soluble myofibrillar fraction. The content of essential aminoacids valine and isoleucine is significantly lower in Coregonus lavaretus L. of Lake Umbozero compared to Coregonus lavaretus L. of Lake Lovozero. Perhaps the lack of these essential aminoacids will lead to the violation of metabolism in the studied fish from Lake Umbozero. The ratio of alkaline aminoacids to acidic ones in the muscle tissue of whitefish from Lake Umbozero is less than that of whitefish from Lake Lovozero. During whitefish puberty the overall pool of essential free aminoacids decreases, the pool is in inverse proportion to content of protein aminoacids.

Keywords

• Coregonus lavaretus
• free aminoacids
• associated aminoacids
• essential aminoacids
• Lake Umbozero
• Lake Lovozero