An efficient <i>i</i>-GONAD method for creating and maintaining lethal mutant mice using an inversion balancer identified from the C3H/HeJJcl strain

Satoru Iwata; Takahisa Sasaki; Miki Nagahara; Takashi Iwamoto

doi:10.1093/g3journal/jkab194

G3: Genes, Genomes, Genetics (Jun 2021)

An efficient <i>i</i>-GONAD method for creating and maintaining lethal mutant mice using an inversion balancer identified from the C3H/HeJJcl strain

Satoru Iwata,
Takahisa Sasaki,
Miki Nagahara,
Takashi Iwamoto

Affiliations

Satoru Iwata: ORCiD; Center for Education in Laboratory Animal Research, Chubu University, Kasugai, Aichi 487-8501, Japan
Takahisa Sasaki: Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, Kasugai, Aichi 487-8501, Japan
Miki Nagahara: Center for Education in Laboratory Animal Research, Chubu University, Kasugai, Aichi 487-8501, Japan
Takashi Iwamoto: Center for Education in Laboratory Animal Research, Chubu University, Kasugai, Aichi 487-8501, Japan

DOI: https://doi.org/10.1093/g3journal/jkab194
Journal volume & issue: Vol. 11, no. 8

Abstract

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AbstractAs the efficiency of the clustered regularly interspaced short palindromic repeats/Cas system is extremely high, creation and maintenance of homozygous lethal mutants are often difficult. Here, we present an efficient in vivoiIn(6)1Jin vitroTprkbIn(6)1JIn(6)1JMitfTprkb

Published in G3: Genes, Genomes, Genetics

ISSN: 2160-1836 (Online)
Publisher: Oxford University Press
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General): Genetics
Website: https://academic.oup.com/g3journal

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