Cancer Management and Research (Nov 2018)
Osthole sensitizes with radiotherapy to suppress tumorigenesis of human nasopharyngeal carcinoma in vitro and in vivo
Abstract
Lin Peng1,*, Yi-Teng Huang2,*, Jian Chen3, Yi-Xuan Zhuang3, Fan Zhang4, Jiong-Yu Chen4, Li Zhou5, Dong-Hong Zhang6 1Clinical Laboratory, Cancer Hospital of Shantou University Medical College, Shantou 515031, People’s Republic of China; 2Health Care Center, The First Affiliated Hospital of Shantou University Medical College. Shantou 515041, People’s Republic of China; 3Department of Radiotherapy, Cancer Hospital of Shantou University Medical College, Shantou 515031, People’s Republic of China; 4Oncological Research Lab, Cancer Hospital of Shantou University Medical College, Shantou 515031, People’s Republic of China; 5Department of Gynecological Oncology, Cancer Hospital of Shantou University Medical College, Shantou 515031, People’s Repulic of China; 6Department of Cardiology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, Zhejiang, People’s Repulic of China *These authors contributed equally to this work Background: Radiotherapy is one of the most comment and useful treatment for nasopharyngeal carcinoma (NPC), but the radioresistance remains a major obstacle. Osthole, a natural coumarin derivative, has been shown to have anti-tumor and anti-inflammatory activity. However, the relationship between osthole and NPC treatment, especially for radiotherapy, is still elusive. Methods: Osthole with or without X ray radiotherapy treated with CNE2 cells, a human EC cell line. Cell viability, proliferation, migration and apoptosis were measured by MTT, colony formation, Annexin V/PI double staining, Transwell assay, respectively. NPC tumor models were established on BALB/c nude mice by subcutaneously injection of CNE2 cells and the effect of osthole and radiotherapy on tumor growth in vivo was studied. Results: We found that in a dose-dependent manner, osthole could individually, and synergistically with radiotherapy, reduce NPC cell (CNE2) viability, proliferation, migration, and invasion, and induce apoptosis, respectively. This effect of anti-tumor growth and induction of apoptosis was further confirmed in mice induced by subcutaneously injection with CNE2 cells and following treated with osthole or/and radiation. Conclusion: Osthole increases the effect of radiotherapy on anti-human nasopharyngeal cancer. Keywords: osthole, radiotherapy, human nasopharyngeal carcinoma, tumorigenesis, proliferation, apoptosis
