Comprehensive Proteomic Analysis of Common Bean (<i>Phaseolus vulgaris</i> L.) Seeds Reveal Shared and Unique Proteins Involved in Terminal Drought Stress Response in Tolerant and Sensitive Genotypes

Mayavan Subramani; Carlos A. Urrea; Sowjanya R. Tamatamu; Venkateswara R. Sripathi; Krystal Williams; Lathadevi K. Chintapenta; Antonette Todd; Gulnihal Ozbay

doi:10.3390/biom14010109

Biomolecules (Jan 2024)

Comprehensive Proteomic Analysis of Common Bean (<i>Phaseolus vulgaris</i> L.) Seeds Reveal Shared and Unique Proteins Involved in Terminal Drought Stress Response in Tolerant and Sensitive Genotypes

Mayavan Subramani,
Carlos A. Urrea,
Sowjanya R. Tamatamu,
Venkateswara R. Sripathi,
Krystal Williams,
Lathadevi K. Chintapenta,
Antonette Todd,
Gulnihal Ozbay

Affiliations

Mayavan Subramani: Molecular Genetics and Epigenomics Laboratory, College of Agriculture, Science and Technology (CAST), Delaware State University, Dover, DE 19901, USA
Carlos A. Urrea: Panhandle Research Extension and Education Center, University of Nebraska, 4502 Avenue I, Scottsbluff, NE 69361, USA
Sowjanya R. Tamatamu: Center for Molecular Biology, Alabama A&M University, Normal, AL 35762, USA
Venkateswara R. Sripathi: Center for Molecular Biology, Alabama A&M University, Normal, AL 35762, USA
Krystal Williams: Molecular Genetics and Epigenomics Laboratory, College of Agriculture, Science and Technology (CAST), Delaware State University, Dover, DE 19901, USA
Lathadevi K. Chintapenta: Biology Department, College of Arts and Sciences (CAS), University of Wisconsin-River Falls, River Falls, WI 54022, USA
Antonette Todd: Molecular Genetics and Epigenomics Laboratory, College of Agriculture, Science and Technology (CAST), Delaware State University, Dover, DE 19901, USA
Gulnihal Ozbay: Department of Agriculture and Natural Resources, Delaware State University, 1200 North DuPont Highway, Dover, DE 19901, USA

DOI: https://doi.org/10.3390/biom14010109
Journal volume & issue: Vol. 14, no. 1
p. 109

Abstract

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This study identified proteomic changes in the seeds of two tolerant (SB-DT3 and SB-DT2) and two sensitive (Merlot and Stampede) common bean genotypes in response to terminal drought stress. Differentially expressed proteins (DEPs) were abundant in the susceptible genotype compared to the tolerant line. DEPs associated with starch biosynthesis, protein–chromophore linkage, and photosynthesis were identified in both genotypes, while a few DEPs and enriched biological pathways exhibited genotype-specific differences. The tolerant genotypes uniquely showed DEPs related to sugar metabolism and plant signaling, while the sensitive genotypes displayed more DEPs involved in plant–pathogen interaction, proteasome function, and carbohydrate metabolism. DEPs linked with chaperone and signal transduction were significantly altered between both genotypes. In summary, our proteomic analysis revealed both conserved and genotype-specific DEPs that could be used as targets in selective breeding and developing drought-tolerant common bean genotypes.

Published in Biomolecules

ISSN: 2218-273X (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: https://www.mdpi.com/journal/biomolecules

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