Creation of Antibody Immunoassay Test Systems for Indication of Enteropathogenic Yersinia

Abstract

3 immunoassay test systems based on antibodies obtained as a result of immunization of rabbits with one of the antigens: disintegrated membranes (DM) Y. enterocolitica, DM Y. pseudotuberculosis and lipopolysaccharide Y. pseudotuberculosis were created. The best diagnostic properties were possessed by a test system based on antibodies to Y. pseudotuberculosis DM. It showed high titers of antibodies to various serovars of Y. pseudotuberculosis (1:25600-1:51200), incl. to microbe strains isolated from farm animals, and low titers to Y. enterocolitica (1:200). In the accumulation media with pig excrements seeded with Yersinia, the experimental test system detected Y. pseudotuberculosis already on the 3rd day of “cold enrichment” provided that 50 or more Yersinia cells per ml were inoculated into the accumulation medium together with excrements.