An updated suite of viral vectors for in vivo calcium imaging using intracerebral and retro-orbital injections in male mice

Sverre Grødem; Ingeborg Nymoen; Guro Helén Vatne; Frederik Sebastian Rogge; Valgerður Björnsdóttir; Kristian Kinden Lensjø; Marianne Fyhn

doi:10.1038/s41467-023-36324-3

Nature Communications (Feb 2023)

An updated suite of viral vectors for in vivo calcium imaging using intracerebral and retro-orbital injections in male mice

Sverre Grødem,
Ingeborg Nymoen,
Guro Helén Vatne,
Frederik Sebastian Rogge,
Valgerður Björnsdóttir,
Kristian Kinden Lensjø,
Marianne Fyhn

Affiliations

Sverre Grødem: Center for Integrative Neuroplasticity, Department of Bioscience, University of Oslo
Ingeborg Nymoen: Center for Integrative Neuroplasticity, Department of Bioscience, University of Oslo
Guro Helén Vatne: Center for Integrative Neuroplasticity, Department of Bioscience, University of Oslo
Frederik Sebastian Rogge: Center for Integrative Neuroplasticity, Department of Bioscience, University of Oslo
Valgerður Björnsdóttir: Center for Integrative Neuroplasticity, Department of Bioscience, University of Oslo
Kristian Kinden Lensjø: Center for Integrative Neuroplasticity, Department of Bioscience, University of Oslo
Marianne Fyhn: Center for Integrative Neuroplasticity, Department of Bioscience, University of Oslo

DOI: https://doi.org/10.1038/s41467-023-36324-3
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 13

Abstract

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Genetically encoded Ca2+ indicators (GECIs) are used to measure neural activity. Here, authors screen GECI constructs for suitability with systemic injections and soma-targeting, and modify a soma-targeting peptide for improved expression rate.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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