Differential Expressions of G6PD and Alkaline Phosphatase Isozymes Associated with Ontogeny and Air-breathing Transition in Channa punctate

Abstract

Differential expressions of glucose-6-phosphate dehydrogenase (G6PD) and alkaline phosphatase (ALP) isozymes which are associated with distinct ontogenetic and developmental changes have been monitored using native PAGE. Prehatching organogenesis involving ectoderm and mesoderm coincided with temporal expression of G6PD-6a, G6PD-6b and ALP-2a isozymes. Abrupt expression of ALP-3 isozyme and silencing of G6PD-6b occurred as late as 14-15 day post-hatching at the time of the transition to air-breathing. These results demonstrate that repertory of G6PD and ALP isozymes in C. punctata is modified to meet the bimodal breathing requirements related to glucose and lipid metabolism despite quite advanced stage of development. Some specific features in the distribution of isozymes of the adult tissue have also been documented.