Nature Communications (Jan 2021)
Single-cell transcriptomic reveals molecular diversity and developmental heterogeneity of human stem cell-derived oligodendrocyte lineage cells
- Xitiz Chamling,
- Alyssa Kallman,
- Weixiang Fang,
- Cynthia A. Berlinicke,
- Joseph L. Mertz,
- Prajwal Devkota,
- Itzy E. Morales Pantoja,
- Matthew D. Smith,
- Zhicheng Ji,
- Calvin Chang,
- Aniruddha Kaushik,
- Liben Chen,
- Katharine A. Whartenby,
- Peter A. Calabresi,
- Hai-Quan Mao,
- Hongkai Ji,
- Tza-Huei Wang,
- Donald J. Zack
Affiliations
- Xitiz Chamling
- Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine
- Alyssa Kallman
- Department of Genetic Medicine, Johns Hopkins University School of Medicine
- Weixiang Fang
- Department of Biostatistics, Johns Hopkins Bloomberg School of Public Health
- Cynthia A. Berlinicke
- Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine
- Joseph L. Mertz
- Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine
- Prajwal Devkota
- Department of Computer Science, University of Miami
- Itzy E. Morales Pantoja
- Department of Neurology, Johns Hopkins University School of Medicine
- Matthew D. Smith
- Department of Neurology, Johns Hopkins University School of Medicine
- Zhicheng Ji
- Department of Biostatistics, Johns Hopkins Bloomberg School of Public Health
- Calvin Chang
- Department of Biomedical Engineering, Johns Hopkins School of Medicine
- Aniruddha Kaushik
- Department of Mechanical Engineering, Johns Hopkins University
- Liben Chen
- Department of Mechanical Engineering, Johns Hopkins University
- Katharine A. Whartenby
- Department of Neurology, Johns Hopkins University School of Medicine
- Peter A. Calabresi
- Department of Neurology, Johns Hopkins University School of Medicine
- Hai-Quan Mao
- Department of Biomedical Engineering, Johns Hopkins School of Medicine
- Hongkai Ji
- Department of Biostatistics, Johns Hopkins Bloomberg School of Public Health
- Tza-Huei Wang
- Department of Biomedical Engineering, Johns Hopkins School of Medicine
- Donald J. Zack
- Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins University School of Medicine
- DOI
- https://doi.org/10.1038/s41467-021-20892-3
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 20
Abstract
Brain myelinating oligodendrocytes are rare and difficult to isolate, which has limited data on their development. Here the authors develop a reporter for scalable purification of human pluripotent stem cell derived oligodendrocyte lineage cells, and use this to map differentiation using single cell RNA-sequencing,
